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What is restriction enzyme mapping?

Restriction enzyme mapping is a powerful tool for the analysis of DNA. This technique relies on restriction endonucleases, hundreds of which are now available, each one recognizing and reproducibly cleaving a specific base pair (bp) sequence in double-stranded DNA thus generating fragments of varying sizes.

What is restriction mapping in cloning?

29 Restriction Mapping At some point in a cloning project it will be necessary to construct a restriction map of a plasmid. This will involve manipulating restriction digest fragments into a circular map. Once you have done this a number of times and develop a “feel” for the process, mapping will be almost intuitive.

What is the restriction mapping of 36 plasmid prit457?

Restriction Mapping 36 Plasmid pRIT457 was cut with BamHI, HindIII, and PstI. From the data below, determine the map. BamHI 5.1 4.5 3.8 HindIII 8.1 5.3 PstI 13.4

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