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How is pmel-1 TCR expressed in B16 melanoma?

A retroviral vector was constructed encoding the pmel-1 TCR genes targeting the B16 melanoma antigen, gp100. Transduction of C57BL/6 lymphocytes resulted in efficient pmel-1 TCR expression. Lymphocytes transduced with this retrovirus specifically recognized gp100-pulsed target cells as measured by interferon-gamma secretion assays.

Why is the pmel-1 T cell receptor transgenic mouse an ideal model?

Rahul Roychoudhuri, … The pmel-1 T cell receptor transgenic mouse has been extensively employed as an ideal model system to study the mechanisms of tumor immunology, CD8 + T cell differentiation, autoimmunity and adoptive immunotherapy.

How to evaluate the efficacy of TCR gene therapy for melanoma?

To evaluate the efficacy of TCR gene therapy, a murine treatment model was developed. A retroviral vector was constructed encoding the pmel-1 TCR genes targeting the B16 melanoma antigen, gp100. Transduction of C57BL/6 lymphocytes resulted in efficient pmel-1 TCR expression.

How was the pmel-1 mouse generated?

The pmel-1 mouse was generated using two transgenic vectors, which contained variable domains of the endogenous TCR as described previously [7] ( Figure S1 ). To design an efficient breeding scheme with other transgenes, it is essential to identify the zygosity status of the pmel-1 transgene.

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