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What is the vector size of pmecs phage?

Map of pMECS phage display vector of 4,510 bp. The lac promoter/operator (Plac) and the secretion signal sequence (pel B) upstream of the VHH insertion site and the hemagglutinin (HA), His tag (His) followed by the gene III of M13 6 … Content may be subject to copyright.

What is a display phagemid vector?

Phagemid display vector. A “typical” phagemid display vector contains origins of replication for double-stranded DNA and ssDNA synthesis (plasmid and filamentous phage origins), an antibiotic resistance gene providing selection of transformed bacteria and a fusion gene under the control of a regulated promoter.

What is the best vector for phage display library preparation?

The pMECS vector was used to construct phage display library. The pcDNA3.1 vector (V790-20, invitrogen, USA) was used for eukaryotic expression of the nanobodies. pLenti-EGFP-luciferase expressing plasmid and gag/pol plasmid were used to prepare SARS-CoV-2 pseudoviruses.

How do you convert a phagemid vector to infective phage?

Phagemid vector is converted into infective phage by superinfection of phagemid-bearing cells with helper phage, as described in the text. Reprinted with permission from Ref. 21.

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