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Exogenous and Endogenous Phosphoethanolamine …
(C) Uptake of NPN (upper panel) and PI (lower panel) by PAO1 or PA14 cells containing pME6032, pMEmcr-1, or pMEeptA, reported as fluorescence emission relative to the empty plasmid controls. In all the assays, strains were cultured in the presence of 0.5 mM IPTG.
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Interference with Pseudomonas aeruginosa Quorum …
Synchronized adult C. elegans hermaphrodites fed with PAO1(pME6032, pBBR1-MCS5), containing two empty plasmids, were killed almost completely after 4 days of incubation.
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Transcriptome profiling defines a novel regulon …
Dec 01, 2009 · PAO1 (pME6032), PAO1 (pME6032- mexT) and PAO1 ΔmexEF (pME6032- mexT) were grown at 37°C shaking (150 r.p.m.) in 20 ml of LB medium supplemented with tetracycline in 100-ml culture flasks. At an OD 600 of 0.5, cell cultures were mixed with Qiagen RNAprotect Bacteria Reagent (Ratio of 1:2) to stabilize RNA.
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MexT modulates virulence determinants in Pseudomonas
Oct 01, 2009 · A mexEF deletion mutant was constructed in the wild-type PAO1 to yield strain PAO1ΔmexEF. pME6032-mexT was introduced into PAO1 and this PAO1ΔmexEF mutant. No significant difference in growth rates was observed between the strains (data not shown).
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pME6032 plasmid - $216.00
pME6032 plasmid can be found in many hosts by using pVS1 and p15A replicas, and can express the protein well with the strong promoter of Tac.
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Intercepting second-messenger signaling by rationally
Jul 21, 2020 · The plasmid pME6032-H6-SUMO-CSP2 ... were verified by DNA sequencing before transformation into E. coli Rosetta for peptide production or P. aeruginosa PAO1 (UJP505). Protein and Peptide Expression and Purification. The peptides CSP1 and CSP2 as well as H 6-CleD were produced using the following protocol.
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(PDF) Positive Control of Swarming, Rhamnolipid …
The value obtained for PAO1/pME6032 (1.21 ⫾ 0.04 g/ml) was set at 100%. were done after separation by normal- or reverse-phase thin-layer chromatog- raphy (silica gel 60 F254, or RP-18 F245; Merck), respectively. E. coli AHL biosensor strains with reporter plasmid pSB1075 (for 3-oxo-C12-HSL detection) or pSB536 (for C4-HSL detection) were ...
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Mutational analysis of the essential lipopolysaccharide
Jul 09, 2020 · (A) Growth curves and (B) plating efficiency of the RecA-deficient P. aeruginosa lptH conditional mutant (PAO1 araCP BAD lptH ΔlptH ΔrecA) carrying the empty plasmid pME6032, pME6032 with wild ...
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Oct 01, 2015 · (B–D) Growth of P. aeruginosa PAO1 with pME6032, P AO-F with pME6032, PAO-F with pME6032- icmF3 in succinate minimal medium (B) , succinate minimal medium supplemented with 50 µ M FeCl 3 (C ...
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Frontiers | Exogenous and Endogenous …
Colistin represents a last-line treatment option for infections caused by multidrug resistant Gram-negative pathogens, including Pseudomonas aeruginosa. Colistin resistance generally involves the modification of the lipid A moiety of lipopolysaccharide (LPS) with positively charged molecules, namely phosphoethanolamine (PEtN) or 4-amino-4-deoxy-L-arabinose (Ara4N), …
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