Keyword Analysis & Research: pme6032
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Intercepting second-messenger signaling by rationally
Jul 21, 2020 · The plasmid pME6032-CSP2 (pTP33) was constructed by inserting the amplified DNA fragment of CSP2 into the EcoRI and SacI restriction sites of pME6032. Primers prTP56 and prTP57 were used to generate the CSP2 fragment. The plasmid pME6032-H6-MBP-CSP2-0 (pTP18) was constructed using a restriction-free cloning method.
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Shuttle vector pME6032 - UniProt
Taxonomy - Shuttle vector pME6032 (SPECIES) ))) Format. Mnemonic i-Taxon identifier i: 390901: Scientific name i: Shuttle vector pME6032: Taxonomy navigation › vectors. Terminal (leaf) node. Common name i-Synonym i-Rank i ...
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T6SS translocates a micropeptide to suppress STING
Oct 08, 2021 · Plasmids pME6032-tssS-vsvg was constructed for protein secretion assay. Briefly, primers tssS-F-EcoRI and tssS-R-BglII were used to amplify the tssSgene from Yptb genomic DNA. The PCR products of tssSwere digested with EcoRI/BglII and inserted into the EcoRI/BglII site of pME6032-vsvgto generate pME6032-tssS-vsvg.
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Mutational analysis of the essential lipopolysaccharide
Jul 09, 2020 · The wild-type and mutant alleles of lptH were individually cloned into the vector pME6032 under the control of an isopropyl-β- d -thiogalactoside (IPTG)-inducible promoter, in order to investigate...
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Optimization of phenazine-1-carboxylic acid production …
Up to12%cash back · Feb 13, 2010 · The strain M18GQ harboring pME6032Phz produced substantially more PCA, making this transformed strain a promising one for industrial PCA production. Designing a suitable fermentation medium is crucial for exploiting the improved productivity of PCA in strains harboring the pME6032Phz plasmid.
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Cloning Should Be Simple: Escherichia coli DH5α …
Sep 08, 2015 · In vivo DNA assembly and cloning in E.coli DH5α. (A) E.coli DH5α-mediated DNA assembly involves only two basic steps: 1) preparation of fragments with homologous ends and 2) introduction of the fragments into competent cells. This approach minimizes the time and reagents required for DNA assembly in comparison to other common methods, which contain …
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Addgene: Vector Database - pBR322
Size is 4362 for USB. Promoter P1 is artificially created by the ligation of two different DNA fragments to create pBR322. Promoter P2 in the same region as P1, but it is on the opposite strand and initiates transcription in the direction of the tetracycline resistance gene. Promoter P3 is the natural promoter for the beta-lactamase gene.
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Dittman, R. A.; Marks, V. 1983-01-01. Management Information System, MIS, provides Life Sciences Projects Division at Johnson Space Center with automated system for project managment. MIS utilizes Tektronix 4027 color graphics display terminal and form-fillout capability. User interface with MIS data base is through series of forms. MIS On-Line.
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(PDF) The Diguanylate Cyclase HsbD Intersects with the
Oct 28, 2016 · Occurrence of PA3343 (hsbD) in Pseudomonas strains and its relation with the flagellar genes reorganization in P. aeruginosa. (A) Evolutionary relationships of Pseudomonas taxa carrying hptB ...
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