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What is PMAL-C2X plasmid?

pMal-c2X Plasmid Description. PMAL system is an efficient protein fusion expression and purification system. The pMAL vector contains the malE gene of the Escherichia coli that encodes the Maltose Binding Protein (MBP). The downstream polyclonal loci are convenient for the insertion of the target gene and express the fusion protein with MBP on...

What is pmal-p2 series?

pMAL-p2 series contains the normal malE signal sequence, which directs the fusion protein through the cytoplasmic membrane. pMAL-p2 fusion proteins capable of being exported can be purified from the peri- plasm. Between the malE sequence and the polylinker there is a spacer sequence coding for 10 asparagine residues.

What does the pmal vector contain?

The pMAL vector contains the malE gene of the Escherichia coli that encodes the Maltose Binding Protein (MBP). The downstream polyclonal loci are convenient for the insertion of the target gene and express the fusion protein with MBP on the N end.

What is the replacement for the pmal™ protein fusion and purification system?

This product has been replaced by the NEBExpress® MBP Fusion and Purification System ( NEB #E8201 ), which offers enhanced performance. The pMAL™ Protein Fusion and Purification System requires a cloned gene be inserted into a pMAL vector down-stream from the malE gene, which encodes maltose-binding protein (MBP).

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