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Frequently Asked Questions

What is a restriction map?

Restriction mapping is a physical mapping technique which is used to determine the relative location of restriction sites on a DNA fragment to give a restriction map. Restriction enzymes are endonucleases that recognize specific sequences on DNA and make specific cuts. How do you draw a restriction enzyme?

How do you do a restriction map on a DNA sequence?

Restriction Map Generator. This tool analyzes a DNA sequence to identify Restriction Enzyme Sites and generate a comprehensive map overview of their locations within the DNA sequence. Enter a DNA sequence in the box below to analyze the sequence for restriction sites and generate a restriction map.

How to make a restriction map of a fragment?

As a first step, you construct a restriction map of the fragment using the enzymes Sma I and Hin dIII. Below is shown an agarose gel of the appropriate digests. Draw a restriction map of the fragment and show the distances, in base pairs, between the Hin dIII, Sma I, and Eco RI sites.

What is a 1000 bp Eco RI restriction fragment?

A 1000 bp Eco RI restriction fragment contains a gene you are interested in. As a first step, you construct a restriction map of the fragment using the enzymes Sma I and Hin dIII. Below is shown an agarose gel of the appropriate digests.

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