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Frequently Asked Questions

What is restriction mapping?

Restriction mapping is a process used to map an unknown segment of DNA by cutting the DNA into pieces with specific restriction enzymes and then identifying the locations of the breakpoints. The enzymes are generally the endonucleases which cut the DNA at specific sites. For performing restriction mapping first of all we need a sample DNA sequence.

How to make a restriction map of a fragment?

As a first step, you construct a restriction map of the fragment using the enzymes Sma I and Hin dIII. Below is shown an agarose gel of the appropriate digests. Draw a restriction map of the fragment and show the distances, in base pairs, between the Hin dIII, Sma I, and Eco RI sites.

What is the restriction-modification system?

The combination of restriction endonuclease and methylase is termed the "restriction-modification" system. Since different bacterial strains and species have potentially different R/M systems, their characterization has made available hundreds of endonucleases with different sequence specific cleavage sites.

How do you draw a genetic map from a plasmid?

Working a DNA into mapping is done using restriction endonuclease enzymes that are found in bacteria, to cut the DNA into fragments. These enzymes cut specific recognition sites of a DNA molecule which may result in a sticky end i.e. over hangs using EcoR1 in the 5’G-AATTC 3′.

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