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What is this topflash construct?

The idea for the construct is from Hans Clevers lab, who designed the original TOPflash. This is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold (activation by phosphorylation mutants of beta-catenin).

What are the best alternate versions of the Flash?

Top 10 Alternate Versions Of The Flash 1 Bizarro Flash 2 Kingdom Come Flash (Wallace West) 3 Red Death 4 Johnny Quick - Crime Syndicate 5 The Crash - Earth C 6 Earth-11 Female Flash 7 Nazi Speedster -Earth 10 8 Accelerated Man - Flash of Earth-19 9 Danica Williams - Beyond Flash

How many TCF/LEF sites does the M50 Super 8x topflash contain?

Note: This plasmid was published as M50 Super 8x TOPFlash, but the plasmid actually contains 7 TCF/LEF sites. These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

Who is the fastest man alive on the Flash?

For the first few seasons, it was all about Barry Allen's nickname of being the Fastest Man Alive being challenged by others — many from alternate Earths. Finally, Flash developed some non-speedster villains to break up the monotony, but by that time, there had been numerous speedy heroes and villains show up in Central City.

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